An improved filtration procedure for measuring opiate receptors in small regions of rat brain.

TitleAn improved filtration procedure for measuring opiate receptors in small regions of rat brain.
Publication TypeJournal Article
Year of Publication1982
JournalJournal of neurochemistry
Volume39
Issue6
Pagination1751-4
ISSN0022-3042
Abstract

A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 micrograms of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

URLhttp://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=1982&volume=39&issue=6&spage=1751
Short TitleJ Neurochem
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